畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (11): 1844-1850.doi: 10.11843/j.issn.0366-6964.2014.11.015

• 预防兽医 • 上一篇    下一篇

1株H12N7亚型禽流感病毒全基因组测定及遗传演化分析

谭丹1,2,崔鹏飞3,黄建龙1,王昌建1,李文辉3,范仲鑫1,张芳3,何世成1,唐小明1,刘道新1*,邓国华3*   

  1. (1.湖南省动物疫病预防控制中心,长沙 410014;2.长沙市动物疫病预防控制中心,长沙 410013;3.中国农业科学院哈尔滨兽医研究所农业部动物流感重点开放实验室/兽医生物技术国家重点实验室,哈尔滨150001)
  • 收稿日期:2014-03-24 出版日期:2014-11-23 发布日期:2014-11-23
  • 通讯作者: 邓国华,E-mail:dgh1971@163.com;刘道新,E-mail:xdl64@tom.com
  • 作者简介:谭丹(1987-),女,湖南隆回人,硕士研究生,主要从事流感病毒分子生物学研究,E-mail:tandan198709@163.com
  • 基金资助:

    科技部专项

Genetic Analysis of the Entire Genome of One H12N7 Subtype Avian Influenza Virus

TAN Dan1,2,CUI Peng-fei3,HUANG Jian-long1,WANG Chang-jian1,LI Wen-hui3,FAN Zhong-xin1,ZHANG Fang3,HE Shi-cheng1,TANG Xiao-ming1,LIU Dao-xin1*,DENG Guo-hua3*   

  1. (1.Hunan Provincial Animal Disease Prevention and Control Center,Changsha 410014,China;2.Changsha Animal Disease Prevention and Control Center,Changsha 410013,China;3.Animal Influenza Laboratory of the Ministry of Agriculture,State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China)
  • Received:2014-03-24 Online:2014-11-23 Published:2014-11-23

摘要:

2011年冬对湖南环洞庭湖地区散养鸭群进行禽流感病毒(AIV)感染情况调查时从一鸭场环境粪便拭子中分离一株H12N7亚型AIV,命名为A/Environment /Hunan/S4484/2011(H12N7),对其进行全基因序列测定及分析。结果显示该毒株HA基因裂解位点无多个连续的碱性氨基酸(PQAQDR↓GLF),具有低致病性AIV的特征,对其全基因进行遗传进化分析表明8个基因片段来源非常复杂,与周边国家野禽中分离的毒株相似性很高,可能有共同的祖先来源,同时提示该毒株可能是不同亚型禽流感毒株之间基因交换所形成的重组体。

Abstract:

H12N7 subtype avian influenza virus (AIV) [A/Environment /Hunan/S4484/2011(H12N7)] was isolated from a duck farm environment in Dongting lake of Hunan during the routine epidemiologic surveillance in 2011.The sequence analysis of the hemagglutinin (HA) gene showed that the virus was categorized as low pathogenic AIV,with the typical HA cleavage site of PQAQDR↓GLF.The phylogenic analysis indicated that all 8 segments of A/Environment /Hunan/S4484/2011(H12N7) was derived from the Eurasian lineage,but the source of the 8 gene segments was very complex,and the highly homologous was observed with different subtypes of AIVs in some regions in Asia and Europe in varying degrees and extensively.Our results suggested that the virus may be a multi-origin reassortant virus.

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